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  • br more toxic products triene conjugates

    2020-08-12


    more toxic products (triene conjugates, Schiff bases), which is con-firmed by the corresponding correlation coefficients (Table 5). It should also be noted that for MDA, the correlation coefficients in normal and in cancer pathology have different signs. Normally, an increase in the intensity of the E 64d bands of lipid groups corresponds to a de-crease in the content of MDA. Against the background of pathology, the level of lipoperoxidation end-products increases as the intensity of the 
    absorption bands of lipids and fatty acids increases.
    At the next stage of the study, the patients included in the study were classified using discriminant analysis (Fig. 4A–C). It has been es-tablished that when using only biochemical data for classification, there is a separation between the control group and patients with oncological diseases (Fig. 4A). At the same time, the separation between these groups is quite clear about the 0-0 vertical axis, but it is difficult to
    Table 3
    The biochemical composition of the saliva of patients with endometrial cancer, ovarian cancer and the control group.
    Indicator Endometrial Ovarian Cancer Control (3)
    differentiate between cancer of the ovaries and endometrium. When using only spectroscopic data, a similar picture is observed (Fig. 4B), however, the groups of patients and healthy differ worse than in the case of only biochemical data. The combination of biochemical and spectroscopic data allows to achieve the most complete separation be-tween groups (Fig. 4C), including the differentiation of ovarian and endometrial cancer relative to the horizontal axis.
    Table 4
    The biochemical composition of saliva, depending on the stage of the disease.
    4. Discussion
    According to modern concepts, the basis of the cell membrane is a double layer of phospholipids, which accounts for the intrinsic prop-erties of a liquid crystal inherent in the membrane. Violations of the structure and function of cell membranes at all stages of the disease are the main expression of pathology in conditions accompanied by tox-icosis. At the same time, in the mechanisms of development of mem-brane pathology, hypoxic disorders of cell metabolism play a leading role, which in turn violates the pathways of biological oxidation in tissues. The Krebs cycle and the oxidation of glucose in the pentose shunt system suffer. The main way to obtain energy is anaerobic gly-colysis with the formation of lactic acid as the final product. One of the consequences of the emerging energy deficit is the accumulation of ions and molecular particles, which lead to oxidative damage to the poly-unsaturated fatty acids that make up the lipid composition of mem-branes [71]. In particular, the role of excessive generation of reactive oxygen species is known, which leads to an increase in lipid perox-idation processes on the background of ovarian and endometrial can-cers [72,73]. Oxidative stress appears to play an important role in the development and progression of ovarian cancer with various events in the malignant transformation associated with active forms of oxygen [74–76]. When antioxidants cannot remove reactive oxygen species, oxidative stress increases and mutations in DNA occur [77].
    IR spectroscopy allows you to determine the total level of metabo-lites formed in the process of pathological changes initiated by tox-icosis, and, accordingly, to assess the degree of disturbance of metabolic processes. However, on the IR spectra, we simultaneously record lipids, as well as primary, intermediate, and final lipid peroxidation products. In this connection, an unambiguous interpretation of the observed changes is difficult. Additionally, one should take into account the complex composition of the studied biological fluid and the possibility of summing the signal of different molecules in the resulting absorption spectrum. The proposed method of pre-extraction of lipids allows fo-cusing exclusively on the lipid component of the IR spectrum without the interfering influence of the absorption bands of proteins and nucleic acids. Comparison with the results of biochemical analysis shows that, against the background of more aggressive ovarian tumors, the balance of lipid peroxidation processes shifts towards the final MDA product, while against the background of endometrial tumors, more inter-mediate products (triene conjugates, Schiff bases) accumulate. The combination of biochemical and spectroscopic data makes it possible to most effectively separate patients from the studied groups, which can be potentially informative when building a diagnostic technique using the methods of multivariate analysis of statistical data.
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